earticle

영어

The final bio-metabolites of lipid peroxidation (LPO) such as 4-hydroxynonenal (4-HNE) and malondialdehyde(MDA) have been suggested to mediate the oxidative stress-linked pathological incidences. Natural phytochemicals such aspolyphenolic compounds in green tea have been known in preventing the LPO induced cellular growth inhibition andapoptosis. We investigated that green tea ethanol extracts (GTE) inhibit LPO-induced apoptosis in ECV 304 cells. GTE hadtime- or dose-dependent anti-apoptotic effects as evidenced by changes in cell morphology, MTT assay, DNA fragmentation,LPO production, and the Western blotting for apoptotic expression. In the 4-HNE-induced apoptosis model, GTE 10-20µg/mL decreased cell death through decreasing LPO production. GTE protected 4-HNE induced apoptosis, as evidence withdown regulation of mitochondrial signaling such as cytochrome C and caspase-3 activity. GTE increased bcl2, survivalsignaling protein, compared to 4-HNE alone within 6hr incubation. Since polyphenols in GTE are effective antioxidants inendothelial ECV 304 cells, we suggested that natural polyphenols might be anti-atherosclerotic.