원문정보
초록
영어
In this study, a rapid and efficient concentrating procedure that can be used for detecting viruses in vegetables wasdeveloped. The Sabin strain of poliovirus type 1 was used to evaluate the efficiency of virus recovery. The procedure included:(a) elution with 0.25 M threonine-0.3 M NaCl pH 9.5; (b) polyethylene glycol (PEG) 8000 precipitation; (c) chloroformextraction; (d) 2nd PEG precipitation; (f) RNA extraction; (g) reverse transcription-polymerase chain reaction (RT-PCR)combined with semi-nested PCR. The overall recoveries by elution/concentration were 29.0% from cabbage and 13.7% fromlettuce. The whole procedure usually takes 18 hr. The overall detection sensitivity was 100 RT-PCR units of genogroup IInorovirus (GII NoV)/25 g cabbage and 100 RT-PCR units of GII NoV/10 g lettuce. The virus detecting method developed inthis study should facilitate the detection of low levels of NoV in cabbage and lettuce.
목차
Introduction
Materials and Methods
Results and Discussion
References
