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Development of a Virus Elution and Concentration Procedure for Detecting Norovirus in Cabbage and Lettuce

원문정보

Aerie Moon, Weon Sang Choi, In-Gyun Hwang

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초록

영어

In this study, a rapid and efficient concentrating procedure that can be used for detecting viruses in vegetables wasdeveloped. The Sabin strain of poliovirus type 1 was used to evaluate the efficiency of virus recovery. The procedure included:(a) elution with 0.25 M threonine-0.3 M NaCl pH 9.5; (b) polyethylene glycol (PEG) 8000 precipitation; (c) chloroformextraction; (d) 2nd PEG precipitation; (f) RNA extraction; (g) reverse transcription-polymerase chain reaction (RT-PCR)combined with semi-nested PCR. The overall recoveries by elution/concentration were 29.0% from cabbage and 13.7% fromlettuce. The whole procedure usually takes 18 hr. The overall detection sensitivity was 100 RT-PCR units of genogroup IInorovirus (GII NoV)/25 g cabbage and 100 RT-PCR units of GII NoV/10 g lettuce. The virus detecting method developed inthis study should facilitate the detection of low levels of NoV in cabbage and lettuce.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

저자정보

  • Aerie Moon Department of Biotechnology, Dongguk University, Gyeongju, Gyeongbuk 780-714, Korea
  • In-Gyun Hwang Department of Food Microbiology, Korea Food & Drug Administration, Seoul 122-704, Korea
  • Weon Sang Choi Department of Biotechnology, Dongguk University, Gyeongju, Gyeongbuk 780-714, Korea

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