earticle

영어

To isolate lactic acid bacteria having high exopolysaccharides (EPS) production ability, 50 strains were initiallyisolated from the sourdough. Twenty-one strains formed highly mucoid colonies on the sucrose agar medium, which areindicative of active EPS synthesis. DU-07, DU-10, DU-12, DU-19, and DU-21 produced 11.51±0.167, 13.09±0.193,12.72±0.108, 11.61±0.284, and 13.32±0.094 g/L EPS, respectively, in MRS medium. The isolated strains, DU-10, DU-12,and DU-21, were identified as Enterococcus flavescens, Enterococcus faecium, and Lactobacillus amylovorus, respectively, byusing API 50CHL kit and determining partial sequences of their 16S rDNA. Especially, L. amylovorus DU-21 showed thehighest production of EPS, as well as the highest inhibitory activities against pathogenic (p<0.05). Interestingly, the L.amylovorus DU-21 seem to be endemic to sourdough fermentations, as they have not been isolated from other environments.