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영어

The prescriptions (DB-1−DB-5) were prepared with the active herbal medicines, Acanthopanax senticosus,Glycyrrhiza uralensis, Polygonatum odoratum, and Cichorium intybus. The most active crude polysaccharide fraction (DB-2-3), which was isolated through the fractionation of hot-water extract from DB-2, was significantly reduced by periodateoxidation (52.7 and 63.7%) on intestinal immune system modulating and anticancer activity. When DB-2-3 was furtherfractionated by column chromatographies, DB-2-3IIc-2 showed the most potent activities. In addition, DB-2-3IIc stimulatedthe proliferation of bone marrow cells via Peyer’s patch in dose-dependent pattern by oral administration. The metastasis ofcolon 26-M3.1 lung carcinoma had significantly inhibited in mice fed DB-2-3IIc at 1mg/mouse (43.8%). DB-2-3IIc-2 mainlycontained uronic acid (46.1%) and 42.5% of neutral sugar with a small amount of protein (7.6%), and component sugaranalysis also showed that DB-2-3IIc-2 was composed Ara, Gal, and GalA (molar ratio; 0.50:0.63:1.00). It may be suggestedthat activities of DB-2-3IIc-2 are resulted from pectic polysaccharides containing a polygalacturonan moiety with side chain ofneutral sugars, such as Ara and Gal.