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영어

Abilities of glucosamine and its derivatives (glucosamine-6-sulfate: SGlc-6, glucosamine-2-sulfate: SGlc-2 and glucosamine-6-phsphate: PGlc-6) to inhibit proliferation of MCF-7 human breast cancer cells were evaluated by measuring cell death via induction of apoptosis.
Among them, glucosamine-2-sulfate (SGlc-2) exerted the highest antiproliferative activity in the human breast cancer. SGlc-2 induced significant proliferative inhibition and apoptosis in a dose- and timedependent manner in MCF-7 human cancer cells. Treatment with SGlc-2 induced the increase in caspase-3, -8 and -9 activities, DNA repair enzyme poly-(ADP-ribose) polymerase (PARP) cleavage and pro-apoptotic gene and the decrease in anti-apoptotic genes. Besides,
NF-κB family and -dependent activated genes were down-regulated by SGlc-2. These results indicated that SGlc-2 had a potential against inhibition of growth of MCF-7 human breast cancer cells, which might be associated with induction of apoptosis through NF-κB or - dependent pathway.