earticle

영어

Variable glycosylation of antibodies occurs at Asn297 in the Fc region, specifically terminal galactosylation and sialylation. These variations may affect both pharmacokinetic behavior and effecter function, such as complement-dependant cytotoxicity (CDC).In this study, glucose, as a
carbon source in culture medium, was substituted for the other sugars in an attempt to induce greater in vivo efficacy of recombinant antibodies and increase their potential as therapeutic drugs by altering glycosylation of antibody. Suspension cultures of recombinant CHO cells were grown in protein-free media, and purification and analysis were performed using liquid chromatographic methods. The results demonstrated that glucose and mannose promote cell growth and lactate production, while galactose and fructose were found to be good energy sources for glycosylation. So, it was proven that the more lactate was produced, the less glycosylation of recombinant antibody was progressed. However, there was a synergistic
effect on glycosylation when mannose was used with other sugars.In summary, carbon sources in culture medium can affect glycosylation of recombinant antibodies. These results provide a reference point for the development of therapeutic recombinant antibodies in the industry.