원문정보
초록
영어
In this study, in order to assess the feasibility of phlorotannins isolated from Ecklonia cava
as an inhibitor of melanin formation, we evaluated its inhibitory effects on mushroom
tyrosinase and IBMX-induced melanin formation inhibitory effects in B16F10 melanoma
cell. The ethanolic (EtOH) extract and ethyl acetate (EtOAc) soluble fraction obtained from
E. cava evidenced a marked inhibitory effect on mushroom tyrosinase at a concentration of
50 μg/ml. Repeated column chromatography of the active EtOAc fraction resulted in the
isolation of three phlorotannins. Their structures were elucidated on the basis of
spectroscopic techniques (1D and 2D NMR) and characterized as phloroglucinol (1),
dioxinodehydroeckol (2) and 7-phloroeckol (3), respectively. Among the compounds, 7-
phloroeckol (3) evidenced more potent tyrosinase inhibitory effect with an IC50 value of 0.85
μM than arbutin (IC50 = 243.16 μM) and kojic acid (IC50 = 40.28 μM), which were used as
positive controls. Lineweaver-Burk plots suggest that 7-phloroeckol plays as a noncompetitive
inhibitor against tyrosinase. Furthermore, these compounds were evaluated for their inhibitory effects on IBMX-induced melanin formation in B16F10 melanoma cells.
Treatment with 7-phloroeckol (6.25-100 μM) resulted in a significant inhibition of melanin
production in the melanoma cells. In this study, we suggest that 7-phloroeckol might prove
useful as a novel inhibitor of melanin formation in cosmetic applications.