원문정보
초록
영어
Detection of Staphylococcus aureus binding with immunoassay was conducted by surface Plasmon resonance (SPR) biosensor, and their kinetics were studied. Titanium isopropoxide was employed as a ligand between gold surface and antibody. The kinetic parameters for
Staphylococcus aureus binding with the immunoassays were evaluated quantitatively. The lower limit of detection for the cell was also investigated in the The lower limit of detection for the cell was improved to 103 CFU/ml by application of the ligand. The association rate constants and affinities of the cell by the ligand application were above twice as large as those by the direct assay. The improvement of the lower limit of detection was derived from the enhancement of
binding attraction between the ligand and antibody.