earticle

영어

Protein arrays are very useful tool for the functional and structural studies of the molecular interactions. To make protein arrays, purification of functional recombinant proteins is required, which is time-consuming and labor-intensive process. Moreover, there is a high demand for the ultra-sensitive detection of important protein, which presents in a low concentration. Thus, we combinded dip-pen nanolithography (DPN), which widely used for nanoarray on a surface,
and ribosome display technique for in vitro selection. After fabrication of acrylamide modified oligonucleotide immobilization on a thiol modified glass surface, the products of EGFP-mRNA fusion molecule of cell-free protein synthesis was hybridized with nanopatterned oligonucleotide for the immobilization of target proteins without purification. This new method, which combines eukaryotic cell-free systems and DPN, provide the easy fabrication of functional protein
array at nanoscale without purification step.