earticle

영어

There have been many attempts to develop sensitive and accurate techniques for the detection and diagnosis of pathogenic bacteria using nucleic acid-based technology. To achieve efficient simultaneous detection of seven selected pathogens, we introduced 16-pair electrode
array containing two copies of 7 specific DNA probes as signal and two negative control as noise manufactured by GENEfluidics, Horseradish peroxidase(HRP) and 3,3’,5,5’-Tetramethylbenzidine (TMB) for pathogenic bacteria detection. The signals were obtained by
amperometric voltammety and cyclic voltammetry. The signal-tonoise ratio was calculated as the signal with 16S rRNA divided by the noise without 16S rRNA. After condition optimization of mixed selfassembly monolayer(SAM), blocking, washing, we investigated the limit of detection (LOD) and signal amplification by Gold-shell Magnetites. This platform might have possibility of fast, precise, quantitative, non-labeling, potable, parallel detection system for pathogen.