earticle

영어

Cell chip is becoming a popular tool for monitoring and detecting a drug or other effects. This system was used for monitoring the neurotransmitter chemicals (dopamine) released from a neural cell (PC12). Upon the cells grow on a chip, dopamine can be detected by three-electrode cyclic voltammetry (CV) method. A cyclic voltammogram of PC12 cells grown on collagen coated gold showed that current peaks were linearly increased with increase of the cell number. The anodic and cathodic peaks are the same potential range for both cellular and chemical dopamine, and it is confirmed that potassium and glucose have a stimulatory effect on dopamine production by the cell. Current peak increased with increase of potassium and glucose
concentrations, and the increase was the greatest when potassium and glucose are used together. The sensitivity of detecting can be increased using potassium and glucose stimulation. These results indicate that CV is an effective method for monitoring of dopamine production from PC12 cells. Using other cell lines and evaluation of drug effect (toxic agent) on this cell chip is on going. Acknowledgments: This research was supported by The Nano/Bio Science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Education, Science and Technology (MEST), Ministry of Environment of the Republic of Korea as "The Eco-technopia 21 project" and by Seoul R&BD Program (10816).