earticle

영어

Nanostructured materials such as nanodot can be utilized as nanobioplatform to construct nanobiochip such as cell chip. Owing to the low fabrication costs and simplicity of immobilizing the target material to the substrate, the fabrication of bioplatform has been a fundamental issue for the development of biochip. A biochip is defined as a collection of miniaturized spots composed of biomaterials arranged on a solid substrate that permits many tests to be performed at the same time in order to achieve higher throughput screenings. The fabrication of two-dimensional arrays of biomolecules on surfaces is an essential technology in the development of biochips. Particularly, two-dimensional arrays of peptides have great potential for biosensors,
bioassaying and tissue engineering. In order to utilize nanobioplatform, we fabricated two-dimensional arrays of Au nanodots on ITO glass using thermal evaporation method through very thin nanoporous alumina masks with through-holes. And HeLa cells were attached on Au nanodot arrays coated with synthetic oligopeptide layer by self-assembly. The topography of Au nanodot arrays was investigated in tapping mode of atomic force microscopy (AFM). The morphology of HeLa cells immobilized on Au nanodot arrays coated with synthetic oligopeptide layer was observed by confocal Microscopy with spatial resolution. These results provide novel insight that Au nanodot arrays formed on solid substrate can be potentially applied as nanobioplatform to construct biochip such as cell chip. Acknowledgement: This research was supported by the Nano/Bio science & Technology Program (M10536090001-05N3609-00110) of the Ministry of Education, Science and Technology (MEST), by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST)(2006-05374), and by The Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2008-359-D00003)