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논문검색

바이오센서 및 바이오칩 분야

Human Olfactory Receptor-Functionalized Polypyrrole Nanotube Transistorsfor Bioelectronic Nose

원문정보

Sang Hun LEE, Hyeonseok YOON, Hyun Seok SONG, Oh Seok KWON, Eun Hae OH, ong Hyun LIM, Jyongsik JANG, Tai Hyun PARK

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.190-190
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

It is known that the human olfactory system has remarkable sensitivity and specificity. Since olfactory system plays an important role in recognizing environmental conditions, kinds of olfactory research have been carried out due to its potential application. In recent years, a few significant studies regarding biotechnology-based olfactory sensors have been reported. However, these sensors still have limitations in sensitivity and specificity. In this work, human olfactory receptor (hOR) 2AG1-conjugated carboxylated polypyrrole nantubes (CPNTs) field effect transistor (FET) sensor was used to measure the binding of odorant molecules to specific receptor proteins. hOR2AG1 was expressed in Escherichia coli (E. coli) as a fusion protein
with glutathione-S-transferase (GST)-tag to its N-terminus for efficient expression. Conductive CPNTs were synthesized by copolymerizing pyrrole monomers with intrinsically functionalized P3CA (pyrrole-3-carboxylic acid) monomers. CPNTs were immobilized onto an interdigitated microelectrode array (IDA) via covalent linkages. hOR2AG1 was then immobilized with CPNTs. As a result, the hOR2AG1-conjugated CPNT FET sensor showed highly specific response to a target odorant, amyl butyrate, and gave measurable signal up to concentrations as low as tens of femtomoles. The hOR-conjugated CPNT FET system offers a new direction of the highly sensitive and selective recognition of odorants, and could be expanded to allow the multiplexed
detection of various odorants after a judicious optimization.

키워드

저자정보

  • Sang Hun LEE School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Hyeonseok YOON School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Hyun Seok SONG School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Oh Seok KWON School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Eun Hae OH School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • ong Hyun LIM School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Jyongsik JANG School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.
  • Tai Hyun PARK School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742.

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