earticle

영어

In this study we have developed optical progesterone sensing probe based on modified CdSe/ZnS quantum dot (QDs). Water-soluble quantum dots (MPA-QDs) were used to label anti-progesterone antibody (P4Ab), and the labeling process was characterized by column
purification. The QDs obtained in organic solvent were modified with mercaptopropionic acid (MPA) and became water-soluble. These water-soluble QDs were linked to the progesterone antibody using the coupling reagents ethyl-3-(dimethyl aminopropyl) carbodiimide(EDC) and N-hydroxysuccinimide(NHS). The linking process was shown to be effective by ultra-filter centrifugation and column purification. After comparing the quantities of P4Ab and water-soluble
QDs involved in the linking reaction via column purification, it was found that a molar P4Ab:QDs ratio of 250ng:0.8mg/ml resulted in most of the water-soluble QDs becoming covalently linked to the P4Ab. CdSe/ZnS quantum dots were synthesized and conjugated with progesterone antibody as progesterone optical sensing probe. The P4Ab conjugated CdSe/ZnS probe sensor has high sensitivity and rapid response time, that it can be used to assay the quality of progesterone.