earticle

영어

Olfactory receptors, belonging to G-protein coupled receptor family, are integral membrane proteins composed of seven transmembrane spanning domains. The olfactory receptor protein is thought to be an efficient primary transducer for the protein-based olfactory biosensor which has high selectivity for odorant detection. Since these proteins have complicated structures and strong hydrophobicity, it is important to optimize reconstitution and refolding condition. The human olfactory receptor was expressed at high-level in E. coli as insoluble form and pre-treated with Triton X-100 to remove impurities. The solubilized receptor proteins were reconstituted with artificial lipid membrane by mixing with an optimal detergent. The functionalization of reconstituted olfactory receptor protein in lipid membrane on sensor
chip was observed by surface plasma resonance (SPR). The proteinbased olfactory bioseonsors using olfactory receptors reconstituted with lipid membranes might demonstrate highly selective odorant detection. This reconstitution method can be applied to other sensing
devices such as QCM or FET for the development of highly sensitive olfactory biosensor.