earticle

영어

T lymphocyte adhesion to adhesion molecules on endothelium is important in immune function, cancer metastasis and inflammation. This cell-cell binding is controlled via cell adhesion molecules such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) of endothelial cells and adhesive properties of T-cell which is greatly modified in several diseased conditions. Thus, therapeutic agents that down-regulate leukocyte-endothelial interactions have been focused to markedly regulate the progression of inflammatory responses in a number of in vivo models. Here, we present a protein based microfluidic system that mimics an inflammatory site which occur the binding of leucocytes to endothelium. This system can easily monitor the binding leucocytes to adhesion molecules under various physiological conditions, such as, shear stress and the density of adhesion molecules. We fabricate protein based microfluidic system by immobilization of cell adhesion molecules (ICAM-1, VCAM-1, E-selectin) on the glass surface. The T cell binding to cell adhesion molecules were affected by the height of channel, density of adhesion molecules and shear stress. The maximum binding was observed at 2 dyne/cm2 of shear stress and 20 ug/ml of the density of adhesion
molecule in the case of ICAM-1. This system is easily integrated with SPR as detection method to monitor the dynamics of reactions in real time. Furthermore, this device is useful as a biosensor for clinical diagnostics and high throughput drug screening.