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영어

Microarrays can be used to screen thousands of binding events in a parallel and high throughput fashion, and are of major importance in the detection of a disease and drug discovery. The use of radioisotope (RI) is conventionally regarded as one of the most sensitive detection methods. However, it takes a long time to implement a data analysis for a signal intensity. A glass chip is exposed to an X-ray film or imaging plate and then developed or analyzed by a bioimage analyzer. Radioactive labeling is mainly performed using different RI’s such as 33P and 32P into ATP for a phosphorylation. We reported here on the degree of incorporation of 33P and 32P into a substrate measured by a radio-TLC. In the examined substrate concentration range, the signal
intensity was continually increased up to a high concentration of E. coli malic-kemptide fusion protein as determined by the radio-TLC. The use of this detection method facilitates a rapid data analysis with a high sensitivity.