원문정보
초록
영어
Protein immobilization is a key step for the development of protein biosensor. In recent
years, people have been seeking various immobilization ways aiming at satisfying a
number of standards for excellent performance of a biosensor, among which, gold
binding polypeptide (GBP) based immobilization began to attract people’s attention.
Here, we established a universal detecting platform utilizing the GBP, protein A (SpA)
and protein G (SpG). Kinds of GBP-fusion protein, such as GBP-SpA, SpA-GBP,
GBP-SpG and SpG-GBP, were prepared via recombinant DNA technology. Surface
plasmon resonance (SPR) was applied to investigate the affinity of these proteins with
the gold surface as well as with immunoglobins. Furthermore, electrochemical
impedance spectroscopy was analyzed for the detection optimization of hepatitis B viral
surface antigen [Our work was supported in part by the IT Leading R&D Support
Project from the Ministry of Knowledge Economy through IITA.].