원문정보
초록
영어
Basic biological and medical research continues to provide the building blocks necessary to develop solutions to human health
problems and to preserve and repair the natural environment. In general, kinases and phosphatases are of considerable interest to
researchers involved in a drug discovery and development, because of their role in a wide variety of diseases. In this study, we investigated the feasibility of applying a RI detection technique to a protein kinase assay for the development of a diagnostic biochip. We cloned a protein-fused substrate for protein kinases using an elevating protein. Results of the study indicate that the protein-fused substrate
can be used effectively as a substrate for detecting the activity of protein kinases and it can be a useful tool for a high throughput
screening and for studying enzyme-substrate interactions.