earticle

영어

Iron oxide/carbon black composite (Fe2O3/CB) electrode was prepared and used for NAD(P)H oxidation under very low overpotential (+0.00V vs. Ag/AgCl) without employment of mediators or any chemical treatment. Based on the capability for oxidation of NAD(P)H by Fe2O3/CB electrode, we developed an amperometric biosensor for the detection of NAD(P)H and ethanol using NAD+-dependent alcohol dehydrogenase. Not only the method of sensor construction is very simple, but also it shows good sensing performances such as high sensitivity, stability, and broad detection range. The sensitivity of this system is 2.54 μA mM-1 and the limit of detection (S/N=3) is 10 μM. The linear range was observed between 10 μM and 1000 μM of
NADH (R2=0.993), which is preferable to previous studies. Moreover, when integrating the developed sensor system into dehydrogenasebased sensor system, it also showed good sensing performance. Details will be presented.