earticle

영어

Carbon nanotubes (CNTs) have stimulated intense research activities, leading to remarkable scientific and technological
advances in nanostructured materials. Although CNTs provide extraordinary mechanical, electrical, and thermal properties
for numerous potential applications, it is difficult to process them due to a major drawback, i.e. poor dispersibility.
Recently, the physical gels consisting of ionic liquids (ILs) and CNTs were fabricated by using imidazolium-ion-based ILs
as new dispersants of CNTs. The CNT/IL bucky gel-modified electrodes are important in terms of understanding electron
transfer of biomolecules. We here report the immobilization of organophosphorus hydrolase (OPH) on the multi-walled
carbon nanotube (MWNT)/IL bucky gel electrode for the biosensors. The MWNT/IL bucky gels were formed by grinding
the mixture of MWNTs and 1-butyl-3-methylimidazolium tetrafluoroborate (BMimBF4) with an agate mortar. After the
immobilization of MWNT/IL gel, MWNT/IL/OPH electrode was obtained by immersing the modified GC electrode into
OPH. The OPH immobilized on the CNT/IL bucky gel electrode surface was confirmed by SEM, EDX and UV-vis
spectroscopy analysis. The interactions between ILs and OPHs were evaluated by FT-IR spectroscopy. The
electrochemical properties of MWNT, MWNT/IL, MWNT/OPH, and MWNT/IL/OPH electrode were characterized by
AC impedance and cyclic voltammetry. MWNT/IL/OPH electrode with effective stability and electrocatalytic
performance provides a useful platform for the development of environmental biosensors [This work was supported in part
by the IT Leading R&D Support Project from the Ministry of Knowledge Economy through IITA].