earticle

영어

Genitourinaty infections diseases are known to occur frequently and some of them have high possibility to pass into chronic diseases. However, current treatments are carried out without broad and exact screening of infected pathogens and antibiotic resistances, causing
overdose of multiple antibiotics. For the efficient diagnosis and treatment, we developed a DNA chip for the simultaneous detection of multiple pathogens causing genitourinary infectious diseases. In this study, we selected 14 pathogens which are abundantly found in Koreans,
and known to cause major infections. Through full consideration of their sequence, we settled the condition for multiplex PCR, and the aldehyde glass surface by our novel method, using gold nanoparticles. By using this method, we could increase the immobilization efficiency, and at the
same time, it was able to control the quality of the manufactured DNA chip by silver enhancement. The target samples were fluorescently labeled by using Cy3 labeled primers. Then, the PCR products were applied to the manufactured DNA chip at the optimized hybridization condition. As a result, we could get the correct signals of the single infection and the multiple infections simultaneously. The developed chip is suitable for the point-of-care testing due to its efficiency, convenience, and the eligible cost of money and time.