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The development of porcine cholera marker vaccine is needed for the maintenance of porcine cholera free region. Insect cells are used as host cells for the expression of marker vaccine by baculovirus vector
system. Insect cell lines are able to grow suspension culture and easy to scale up. The use of FBS has several disadvantages and is undesirable in large scale insect cell cultures media. Therefore, high density insect cell culture in serum free medium is prerequisite to produce a marker vaccine with high productivity and safety.
Therefore, it is important to select the optimum serum free medium for host cell line. In this work, two insect cell lines, Hi5 originated from the eggs of Tricoplusiu ni and Sf9 derived from the ovary of Spodopteru frugiperdu, were investigated for their ability to grow in a serum-containing medium and in serum free media in erlenmeyer
flask. An inoculum concentration of 5 x 105 cells/ml results in high growth rates in both Sf9 and Hi 5 cell line. The best medium for the cell growth was sf900Ⅲ SFM and Ex-cell 405 SFM in sf9 and Hi 5cell, respectively. The optimum serum-free media were selected in terms of cell density, and the selected serum free media showed higher cell growth than the medium containing FBS.