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Mesenchymal stem cells are multipotent cells capable of differentiating into various mesenchymal tissues, such as bone, cartilage, fat, tendon and muscle. In order to explore there usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. The expansion of MSCs strongly depends on the culture conditions. In order
to explore usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. The expansion of MSCs strongly depends on the culture conditions like pH, temperature, oxygen, osmolarity, basal medium, serum and other supplements. Carbon dioxide in the gas phase dissolves in the medium, establishes equilibrium with HCO3
− ions, and lowers the pH. The concentration of CO2 is in equilibrium with the sodium bicarbonate in the medium. We focused on effect of gas phase CO2 in the culture of mesenchymal stem cells. Human cord blood MSC, canine bone marrow MSC and canine cord
blood MSCs were cultured in 5% CO2 and 10% CO2 condition. Media such as DMEM, KSFM and CDM 1.0 were used. As result cells cultured in DMEM shown batter growth in 10% CO2 and cells cultured in KSFM or SJ-CDM1.0 shown batter growth in 5% CO2 condition. We conclude
that effect of gas phase CO2 depends on culture medium.