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초록
영어
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious infection of swine, which is classified by the Office International des Epizooties (OIE) as a list of a disease. Envelope glycoprotein E2 is the major neutralizing antigen for CSFV infection. In this study, we describe the expression of recombined CSFV E2 gene by tobacco chloroplast transformation. The E2 gene was cloned into chloroplast targeting vector for high level expression. The recombinant vector was transformed to tobacco plant
by particle bombardment method. Genomic DNA PCR analysis confirmed stable homologous recombination of the E2 gene into the chloroplast genome. And Northern blot analysis confirmed the expression level of mRNA.