earticle

영어

Magnetic nanoparticles isolated from Magnetospirillum sp. AMB-1 can be applied to various therapeutic materials. However, the intracellular uptake pathway of magnetic nanoparticles has not been clearly elucidated yet. In this study, we investigated the intracellular uptake mechanism of magnetic nanoparticles. Magnetic nanoparticles were
delivered to the endothelial progenitor cells (EPC) and their uptake pathways were investigated through various methods. Magnetic nanoparticle internalization into EPCs increased with incubation time in the presence of magnetic nanoparticles in the medium. Cellular uptake of magnetic nanoparticles was inhibited in the low temperature (4℃) and confocal study demonstrated that nanoparticles were internalized via endosome at least within 1 h after incubation. We also studied the exocytosis of magnetic nanoparticles by time-dependent manner. Our results demonstrated that the uptake process of magnetic nanoparticles was dynamic and the magnetic nanoparticles from AMB-1 could be internalized into the EPCs through energy-dependent endocytotic
pathway.