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reserves. There is a need of increased efforts required for the development of new alternative energy sources, which replace crude oil, with less cost and eco-friendly approaches. Bioethanol production from crop wastes is one of the major work progresses by various countries to meet their energy consumption in the last two decades. Delignification is the major problem in using biomass. We have compared acid, alkali and microwave treatment for delignification of rice straw. Alkaline treatment is found to be the most effective delignification process among those studied. Cellulases play a major role in the saccharification of biomass. Based on the preferential pretreatment approach, the suitable cellulase exploration produce authentic results. We have isolated a novel Bacillus amyloliquefaciens K5 strain producing a slightly alkaline pH stable cellulase. This enzyme found to be useful in reducing sugar production from alkali pretreated biomass fermentation. We have obtained a 44% saccharification by initial weight using the commercial Novozyme cellulase on alkali pretreated ricestraw. The results of various pretreatments for rice straw delignification, saccharification with commercial cellulases, media optimization using pretreated rice straw as a carbon source for higher cellulase production by Bacillus amyloliquefaciens K5 will be presented.