earticle

영어

Because of the limited stocks of fossil fuels and the production of greenhouse gas on their combustion, alternative sources of energy have been investigated. And following the 'Low Carbon, Green Growth' as Korea's future vision, we need to find a green energy source. One possible source of biological material for fuel production is microalgae. Investigations were carried out on the uptake of lipid by the green alga Chlorella emersonii. In order to get the high lipid content using C. emersonii, a medium optimization experiment was designed and performed. The effect of media composition on the lipid of C. emersonii was examined by fractional factorial design (FFD) and central composite design (CCD). The media components examined include potassium nitrate, potassium phosphate, magnesium sulphate, ferrous sulphate, and trace metal solution. Trace metal solution was used 1 ml of microelements composed of H3BO3 (2.86 g), MnCl24H2O (2.84 g), ZnSO47H2O (0.22 g), Na2MoO42H2O (0.018 g), CuSO45H2O (0.29 g) in 1000 ml distilled water. All experimental sets were cultivated under 5% CO2 balanced with 95% air and under 60 μE/m2s of light intensity. The results indicated that potassium nitrate, potassium phosphate, and trace metal solution were major factors. The optimum concentrations of potassium nitrate, potassium phosphate, and Trace metal solution were found to be 1.63, 1.06 g/L, and 1.35 ml/L respectively, for lipid production. The optimized media was shown a similar cell concentration although fresh cell weights were 19% higher. The lipid production with optimized medium showed a 30% enhanced production than that with original Watanabe medium.