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환경생물 및 에너지 분야

Cell Growth and Lipid Production of Chlorella minutissima upon Cultivation Conditions in Fed-batch Culture

초록

영어

Cell growth and lipid production of Chlorella minutissima in fed-batch culture was studied. Two carbon resources, such as CO2 and glucose were used to compare autotrophic and heterotrophic cultivate condition. For fed-batch cultivation, the feeding rate of seawater medium containing glucose was decided to be 0.5 ℓ/day of medium containing glucose. Autotrophic with CO2 showed the maximum cell growth of 9 (g-dry wt./L) and total lipid production of 15%. heterotrophic condition with glucose 10 g/ ℓ showed the maximum cell growth of 15 (g-dry wt./L) and total lipid production of 32%. Also, lipid profiles produced by gas chromatography analysis showed that lipid concentration of C17 and C18 composition is each 16%, 38%. This tells that Chlorella minutissima is a suitable bio-resource to produce the biodiesel. Accordingly, this study showed that the fed-batch cultivation with glucose is most effective for the large scale cultivation for biodiesel production with Chlorella minutissima.

저자정보

  • SungHo OH Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • JaeGun HAN Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • JiHye HA Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • Young KIM Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • SeungSeop KIM Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • MyeongHoon JEONG Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • HyangSuk JEONG Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • MinChul KWON Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University,
  • NaYoung KIM Dept. of Biological Engineering College of Engineering, Kangwon National University, Chuchon 200-701, Korea.
  • ShinYoung LEE Dept. of Biological Engineering College of Engineering, Kangwon National University, Chuchon 200-701, Korea.
  • JeongSub CHO DooSan EcoBizNet, Chunchon 200-161, Korea.
  • WonByong YOON Dept. of Food Science & Biotechnology College of Bioscience and Biotechnology, Kangwon National University, Chunchon 200-701, Korea.
  • HyeonYong LEE 1.)Dept. of Biomaterials Engineering College of Bioscience and Biotechnology, Kangwon National University, Chunchon 200-701, Korea, 2.)Research Institute of Bioscience and Biotechnology, Kangwon National University, Chunchon 200-701, Korea.

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