earticle

영어

Conventional biopanning protocol has been applied to explore the peptide sequence having affinity to a target compound starting from a phage-displayed peptide library through a batch type adsorption protocol. The basic principal of biopanning protocol is very similar to the general chromatographic separation which is widely used in bioseparation processes. This study was carried out to improve the efficiency of biopanning protocol based on that similarity of both protocols. The biopanning protocol was automated by using a FPLC equipped with monolithic column which virus particles pass through. This new protocol made it possible to develop “chromatographic library panning protocol” which can search for the specific peptide sequence within a short time compared to the conventional biopanning protocol. Pb2+ has been known to be highly toxic to a majority of living entities and thus classified with other heavy metal ions as endocrine disruptors. The iterative passing of phage particles through the Pb2+-combined monolithic column in FPLC was done to find the peptide sequence having high affinity to Pb2+. Then the screened Pb2+- binding phage particles was sequentially loaded to the other monolithic columns combined with other metal ions (Ni2+, Cu2+, Zn2+, Co2+) to remove the peptide sequence having cross-binding affinity to other metal ions.