earticle

영어

Marine bacteria which possess an alginate degrading activity were isolated from seasoned brown seaweeds with fermented anchovies. Small amount of the liquefied substances was diluted with sterilized water and smeared on M9 alginate agar plate. Among the alginate degrading colonies, a plane, rough, opaque like shape colony was chosen. Analysis of 16S ribosomal DNA sequence and constructed phylogenetic tree revealed that the strain was closest to Caulobacter leidyi, Caulobacter endosymbiont, and Sphingomonas sp. We designated this strain as Marine Bacterium MJ3. Strain MJ3 grew best on the minimal agar plate containing 0.8% sodium alginate as a sole carbon source and showed resistant against several antibiotics. When the strain MJ3 was cultured in the minimal media with alginate addition, the alginate lyase activity occurs in the cell lysate not in the culture soup. It is concluded that the MJ3 engulfs macromolecule alginate into the cell and degraded it to oligo-algininate and mono-alginate as an energy source. Acknowledgement: This work was financially supported by the 2008 Busan Techno Park program and the Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.