earticle

영어

A microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as Bacillus atrophaeus by analyses of 16S rDNA sequences, and named as B. atrophaeus LBH-18. Rice bran and ammonium peptone were the best combination of carbon and nitrogen sources for the production of the CMCase by B. atrophaeus LBH-18. Based on intuitive analysis and statistical calculations using data from an L25(35) orthogonal design experiment with three factors of rice bran, peptone, and initial pH of medium, rice bran was found to be the most important factor for production of the CMCase by B. atrophaeus LBH-18. Optimal concentrations of rice bran and peptone for production of CMCase were found to be 5.0% and 0.5%, respectively. Optimal initial pH of medium and temperature were 7.0 and 30℃. Effect of salts was also investigated using an orthogonal array method. Potassium phosphate from four salts in the medium was found to be the most important factor for production of the CMCase by B. atrophaeus LBH-18. Productivity of the CMCase by B. atrophaeus LBH-18 was about 120 U/ml under optimized conditions.