원문정보
초록
영어
Some Antarctic algae are known to produce ice binding proteins. IBPs are diverse proteins with the capability of inhibiting ice crystal growth against in subzero environments for their survival. Two microalgae, named AnF0046 and AnF0048, were used in this study. To examine the physiological properties, we carried out a comparative analysis on growth with Chlamydomonas reinhardtii. Growth rate of C. reinhardtii is 1.6 and 1.1 fold higher than those of AnF0046 and AnF0048, respectively. AnF0046 and AnF0048 produced 1.4 and 1.7 fold lower amounts of chlorophylls than that of C. reinhardtii. Extraction of genomic DNA was conducted for confirmation of gene existence related to IBPs. The bands on 1-D gel from either AnF0046 or AnF0048 were comparable with that from C. reinhardtii. To search IBP encoding gene, primers were designed based on IBP isoforms produced by Chlamydomonas sp. CCMP681. The primer designed from isoform1 had the most homologous sequence. A single band at 2.5 kbp was found from overlapping fragments purified. Forward and reverse sequences, each with 950 bp, were obtained after sequencing. The two Antartic microalgae, AnF0046 and AnF0048, found to have IBP coding gene.