earticle

영어

Pine needles have long been used as a traditional health-promoting medicinal food in korea. To
investigate the effects of pine(pinus densiflora)needle extracts on hepatic antioxidative system and serum lipid improvement were assessed in rats fed high-cholesterol diet Sprague Dawley rats of 8 week-old were divided into four groups; normal diet group (CON), 0.5% cholesterol diet group (0.5% CHOL), 0.5% cholesterol diet and pine needle extracts prepared by using hot water group (HOT water), and 0.5% cholesterol diet and pine needle extracts prepared by using subsupercritical CO2 group (Sub-supercritical). The serum ALP, AST and ALT activities were
increased by 0.5% cholesterol diet supplementation, but treating with pine needle extracts was
disposed to decrease comparing with 0.5% CHOL group. The SOD activity in liver at 0.5%
cholesterol diet group was decreased than control group while Sub-supercritical group had
significantly higher SOD activity than 0.5% CHOL group. Moreover, groups with pine needle
extracts supplementation had higher level of GPx in liver than 0.5% cholesterol diet group. Serum
total cholesterol and triglyceride contents were lower in CON group compared with 0.5% CHOL
group. Three weeks feeding of hot water and sub-supercritical extract resulted in a significant
lowering of serum triglyceride and lowering tendency of total cholesterol Level. The level of
HDL⁃cholesterol in the 0.5% CHOL group was significantly (p<0.05) reduced compared with
CON group, but it was tended to increase by pine needle extract supplementation. These results
suggest that pine needle extracts increased biological activities. Acknowledgement: This work was supported by Human Resource Training Project for Regional Innovation.