earticle

영어

Effects of glucosamine-6-phosphate (Glc-6-P) on adipocyte differentiation of 3T3-L1 cells were investigated by measuring triglyceride level and leptin and glycerol secretions as indicators of lipid accumulation and gene expressions of CCAAT/enhancer-binding protein-α (C/EBPα), sterol regulatory element-binding protein 1c (SREBP1c), peroxisome proliferator-activated receptor-γ (PPARγ) and Leptin in cultured 3T3-L1 adipocytes. Glc-6-P significantly reduced lipid accumulation, a marker of adipogenesis, in a dose dependent manner. The mRNA expression levels of SREBP1c, C/EBPα, PPARγ and Leptin were markedly downregulated by Glc-6-P. Moreover, Glc-6-P inhibited the protein expression of C/EBPα and PPARγ, the key adipogenic transcription factors. Tumor necrosis factor-α (TNFα) is reported to impair preadipocyte differentiation and induce lipolysis. Glc-6-P markedly reduced mRNA expression level of TNFα. These results suggest that inhibitory effect of Glc-6-P on adipocyte differentiation might be mediated through the down regulated expression of adipogenic transcription factors.