earticle

영어

Chaga mushroom (Inonotus obliquus) has long been used as a folk medicine due to its numerous biological funtions such as antibacterial, anti-allergic, anti-inflammatory and anti-oxidative activities. In the present study, we found that the hot water extract of chaga mushroom activated adipogenesis of 3T3-L1 preadipocytes. The major constituent of the extract was glucose-rich polysaccharides with a molecular mass of 150 kDa. The extract enhanced differentiation of 3T3-L1 preadipocytes, increasing accumulation of lipid droplets in adipocytes in a dose-dependent manner. Immunoblot analysis revealed that the extract increased the expression of adipogenic makers such as PPARg, GLUT4, aP2 as well as accelerated triacylglycerol accumulation that is critical for acquisition of the adipocyte phenotype. In addition, even in the absence of adipogenic stimuli by insulin, the chaga extract strongly induced adipogenesis in 3T3-L1 preadipocytes. Taken together, these results indicate that the chaga extract can be developed as a PPARg agonist for the improvement of insulin resistance mediated with diabetes because ligands for this receptor have emerged as potent insulin sensitizers used in the treatment of type2 diabetes.