원문정보
초록
영어
Butanol has received considerable attention in relation to a possible alternative to gasoline fuel and can be produced by the biological process using Clostridia through acetone-butanol-ethanol (ABE) fermentation. Clostridium beijerinckii is one of the primary butanol producers. To improve the production of butanol in C. beijerinckii, we engineered the strain by random mutagenesis using NTG. Among the NTG-treated cells, we selected 3,000 colonies and transferred to 1 ml P2 medium in each well of 96-well deep plates and cultured. The concentration of butanol of each clone was measured by using gas chromatography. We further selected the mutant strains showing the increase in the butanol production of more than 10%, compared to the wild-type strain, and transferred to 10 ml P2 medium in each 15 ml conical tube and cultured. After two-step screening of 3,000 clones, N005, the selected mutant exhibiting the highest butanol production, showed about 30% increase in the level of butanol, compared to the wild-type strain. When batch cultivation of this mutant strain was carried out in 2 l fermenter containing 1 l P2 medium, the production level of butanol was approximately 14.6 g/l.