원문정보
초록
영어
Klebsiella pneumoniae is a typical microbial strain capable of producing 1,3-propanediol that is a valuable chemical that is used mainly for the synthesis of polymethylene terephalates by polymerization with terephthalates (1). The microbial production of 1,3-PD by K. pneumoniae involves the formation of various by-products which are synthesized through the oxidative pathway. 2,3-Butanediol, one of the main by-products, may serve as an obstacle for obtaining a high purity of 1,3-PD in downstream processes because of its similar boiling point (2). Hence, to eliminate the by-products synthesis, the oxidative branch of glycerol metabolism was inactivated by deleting from the chromosomal DNA the genes involved in the synthesis of by-products. The by-product formation except for acetate was eliminated in the resultant strains, giving the production yield of 0.57 mol/mol which was higher as compared to that of the wild type strain which gave the production yield of 0.47 mol/mol.