earticle

영어

Cyclosporine A (CyA), a cyclic polypeptide metabolite extracted from the fungus, Tolyplocadium inflatum, has been widely used as an immunosuppressive agent in organ transplantation both in human and veterinary medicine. To increase CyA productivity by cyclosporineproducing strain of T. niveum, a foreign bacterial gene, Vitreoscilla Hemoglobin gene (VHb), was transformed via protoplast regeneration, followed by its transcription confirmation using RT-PCR. One T. niveum transformant which stably contained and expressed VHb gene led to a 33.5% increase of CyA productivity comparing with the control strain. Although some protoplast-regenerated T. niveum transformants tend to lose CyA productivity, the optimized protoplast transformation should be an efficient alternative strategy to generate a commercially-valuable, yet metabolite low-producing, fungal species, such as CyA-producing T. niveum.