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유전자 및 대사공학 분야

Novel Pathway-specific Regulator which Binds to the Promoter Region of CYP105D7 which Has the Ability of Converting Daidzein to Hydroxydaidzein in Streptomyces avermitilis

원문정보

Ji-Nu KIM, Eunjung SONG, Eun-Jung KIM, Bo-Rahm KIM, Nahum LEE, Byung-Gee KIM

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.143-143
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초록

영어

than those of daidzein. P450s have the ability of converting daidzein to hydroxydaidzein i.e. 3΄-hydroxy, 6-hydroxy, and 8-hydroxydaidzein. We already found that CYP105D7 (SAV7469) has the specific activity of converting daidzein to hydroxydaidzein. We compared the various strains for the biosynthesis of hydroxydaidzeins. Among strains examined, Streptomyces avermitilis MA4680 highly converted daidzein to orthospecific dihydroxyisoflavones. We investigated recombinant Streptomyces avermitilis strain found to have 3.6 times higher ability than wild type to convert 3΄-hydroxydaidzein. In this report, we found a new pathway-specific regulator which binds to the promoter region of CYP105D7 (SAV7469) in Streptomyces avermitilis by the DNA affinity capture assay method. Since this regulator is well conserved throughout the Streptomyces species, it is expected to have an important role in Streptomyces avermitilis. We made an overexpression mutant, deletion mutant of this regulator and checked the activity of converting daidzein to hydroxydaidzein compared to the wild type strain.

키워드

저자정보

  • Ji-Nu KIM Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.
  • Eunjung SONG Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.
  • Eun-Jung KIM Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.
  • Bo-Rahm KIM Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.
  • Nahum LEE Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.
  • Byung-Gee KIM Dept. of Chemical and Biological Engineering, Seoul National University, Seoul, 151-744.

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