earticle

영어

1,2-Propanediol, also known as propylene glycol, is a major commodity chemical with world market of $ 123.6 millions/yr. In our previous research, we engineered S. cerevisiae strain to produce 1,2-propanediol with tpi1(triosephosphate isomerase) deleted mutant. By introduction of E. coli mgs(methylglyoxal synthase) and gldA(glycerol dehydrogenase) using a multicopy plasmid, 1.11 g/l 1,2-propanediol was achieved with 2%(v/v) ethanol as a major carbon source. In this study, glycerol was used as a sole carbon source under microaerobic condition, much higher 1,2-propanediol productivity was achieved with overexpression of mgs and gldA. The productivity was enhanced when fps1 gene encoding glycerol transporter was overexpressed in S. cerevisiae. As result, we got 3.23 g/l 1,2-propanediol concentration during 144h flask cultivation with 1%(v/v) glycerol as a carbon source.