earticle

영어

Peroxidase catalyzes the oxidation by hydrogen peroxide of a number of substates, which has important roles in plants under oxidative stress. Leyumus chinensis (Chinese leymus) is become as a soil-binding plant to protect soil from desertification. In this study, we analyzed PRX gene of certain model plants (including rice, Arabidopsis thaliana, wheat, and bread wheat), and discussed the homology in term of plant genetics. We found that the diversity among sequences is great, expect of two sets of sequences from wheat and bread wheat (100% and 95%, respectively). Others had less than 33% of homology, even from the same species (HV-AF411228 and HV-AJ003141). So it is said that PRX gene of Chinese leymus which is closely relative with wheat according to genetics, was difficult to orientate in the homology tree written by 20 sequences of model plants. Compared with Chinese leymus EST peroxidase data and HV-DL138603 and TA-AY506502, results suggested that defence-relative EST sequences had higher homology (61%) with wheat, but not CD809225 and other metabolism relative EST sequences. The results are suitable to further study of cloning, analysis and orientation of PRX gene of L. chinensis.