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영어

Aminoglycosides are a group of antibiotics that are used to treat certain bacterial infections. This group of antibiotics includes at least eight drugs, amikacin, gentamicin, kanamycin, neomycin, netilmicin, paromomycin, streptomycin, and tobramycin. UDP-kanosamine (UDP-3-amino-3-deoxyglucose) is a sugar moiety of kanamycin that was highly inhibitory to growth of plant-pathogenic oomycetes and moderately inhibitory to certain fungi and inhibited few bacterial species tested. Biosynthesis of UDP-kanosamine starts with UDPglucose via UDP-3-keto-glucose. Here we have characterized KanC as a UDP-glucose dehydrogenase to form UDP-3-keto-glucose, an intermediate of UDP-kanosamine. We have cloned KanC in expression vector pET32a+ and express the soluble protein. Enzymatic assay was carried out using UDP-glucose as a substrate and NADP+ as a cofactor. The product was analyzed by HPLC and ESI-MS.