earticle

영어

The mpi gene encoding mannose-6-phosphate isomerase (GTMpi) from Geobacillus thermodenitrificans was cloned and expressed in Escherichia coli. GTMpi converted aldose substrates possessing hydroxyl groups oriented in the same direction at the C2 and C3 positions, such as the D- and L-forms of ribose, lyxose, talose, mannose, and allose. D-Lyxose isomerization was optimal at pH 7.0 and 70°C with 1mM Co2+. The half-lives of the enzyme at 60°C, 65°C, and 70°C were 388 h, 73h, and 27 h, respectively. D-Lyxose was converted to D-xylulose by GTMpi with 38% conversion yields after about 3 h, whereas L-ribose was converted to L-ribulose with 29% conversion yields after about 3 h.