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효소공학 분야

Nano-immobilization of the alginate lyase from a marine bacterium Streptomyces sp. ALG-5

원문정보

Ji Young MIN, Mi Hee WOO, Hee Sook KIM, Eun Yeol LEE

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.131-131
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

The alginate lyase gene of Streptomyces sp. ALG-5 was cloned by using PCR, and the alginate lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The recombinant alginate lyase was purified and immobilized onto magnetic nanoparticles. The immobilized alginate lyase was used to hydrolyze alginic polymer, and oligosaccharides of di-, tri-, tetra- and pentasaccharides were produced. The immobilized alginate lyase was efficiently recovered and reused repeatedly by magnetic separation.

키워드

저자정보

  • Ji Young MIN Department of Chemical Engineering, Kyung Hee University
  • Mi Hee WOO Department of Food Science and Biotechnology, Kyungsung University,
  • Hee Sook KIM Department of Food Science and Biotechnology, Kyungsung University,
  • Eun Yeol LEE Department of Chemical Engineering, Kyung Hee University

참고문헌

자료제공 : 네이버학술정보

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