earticle

영어

B-type natriuretic peptide is a cardiac derived peptide hormone. It is secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells. BNP levels are related to the severity of sings and symptoms of heart failure. In this respect, its concentrations have been able to accurately reflect diagnosis, prognosis and differentiate heart failure care pathway. It has emerged as a receptor of BNP, useful and cost-effective biomarkers, anti-BNP scFv antibody development is meaningful. But there is no research about anti-BNP scFv antibody, we try express anti-BNP scFv antibody through Escherichia coli expression. In an attempt to develop, we engineered anti-BNP scFv antibody in pET vector and it is possible to make folded and functional antibody fragments in excellent yield.