earticle

영어

Aureobasidium pullulans has been known as microorganisms that produce a large amount of β-1,3-1,6-glucan (exopolysaccharides) used for health foods and cosmetics. To achieve production of higher EXPs (exopolysaccharides), we have optimized media for EXPs by using OFAT (one factor at a time) method and stastical analyses (Plackett-Burman design and Box-Behnken design). Basal medium were determinred in Czapek-Dox broth, Ashbya gossypii extract and amonium sulfate instead of sodium nitrate. Our study showed that sodium nitrate played an important role in cell growth and EXPs production. The relative importance of medium components for maximum glucan production was eavlauated by using Plackett-Burman design. It was showed that sucrose, amonium sulfate and A. gossypii extract were significant components for EXPs production. Also, on the basis of Plackett-Burman design analysis, Box-Behnken design was carried out to search for optimum medium of EPSs production. As a result of the box-behnken design and response surface methodology, EXPs production of the optimum medium was two or three times higher than one of the basal medium(5.34 g/L).