earticle

논문검색

미생물공학 분야

Production of Psychrophilic Chitinase from Antarctic Microorganism by Recombinant Escherichia coli

원문정보

Se Jong HAN, Heeyong PARK, Dockyu KIM, Il-Chan KIM, Joung Han YIM

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.125-125
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

In order to enhance the production of psychrophilic chitinase, recombinant Escherichia coli Top 10 harboring chitinase gene from Antarctic microorganism KOPRI 22718 was cultivated using flask and jar fermentor. Expression of target protein was performed by Larabinose addition and confirmed by SDS-PAGE and activity test with p-nitrophenyl-N-acetyl-b-D-glucosaminide as a substrate.To increase the cell density and production of enzyme, modified R-medium was tested as a fermentation medium and effect of L-arabinose concentration was also observed. About 28 times higher enzyme production was obtained by using modified R-medium with lactose and 2.5g/L of L-arabinose in fermentor culture than that by using LB medium in flask culture.

키워드

저자정보

  • Se Jong HAN Polar BioCenter, Korea Polar Research Institute, Incheon 406-840, Korea.
  • Heeyong PARK Polar BioCenter, Korea Polar Research Institute, Incheon 406-840, Korea.
  • Dockyu KIM Polar BioCenter, Korea Polar Research Institute, Incheon 406-840, Korea.
  • Il-Chan KIM Polar BioCenter, Korea Polar Research Institute, Incheon 406-840, Korea.
  • Joung Han YIM Polar BioCenter, Korea Polar Research Institute, Incheon 406-840, Korea.

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.