earticle

영어

Optimal conditions for production of the carboxymethycellulase (CMCase) and filterpaperase (FPase) by E. coli pTDL-3 were investigated in a 7L bioreactor using an orthogonal array method. On basis of intuitive analysis and statistical calculations using data, significance of aeration rate on production of the CMCase by E. coli pTDL-3 was higher than that of agitation speed on production of the CMCase as well as for production of the FPase. Optimal agitation speed and aeration rate for the production of CMCase were found to be 200 rpm and 1.0 vvm in a 7 L bioreactor. Productivity of the CMCase by E. coli pTDL-3 from 5.0% (w/v) rice bran and 0.1% (w/v) tryptone under optimized conditions was 544.4 U/ml in a 100 L bioreactor with an inner pressure of 0.2 kgf/cm2.