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미생물공학 분야

Cloning and Expression of Flavin-contatining Monooxygenase Gene in the various Host and Vector System

원문정보

Gui Hwan HAN, Seong Hun IM, Jang Min PARK, Si Wouk KIM

한국생물공학회 한국생물공학회 학술대회 2009 춘계학술대회 및 국제심포지움 2009.04 pp.123-123
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

In the previous study, we reported that indigo was produced by recombinant E. coli harboring a flavin-containing monooxygenase gene. This recombinant E. coli produced 5.6g/L/day of indigo for 5day on the tryptophan medium (0.2% tryptophan, 1% NaCl, 0.5% yeast extract) through fed-batch cultivation. In this study, investigated the expression of fmo gene in the E. coli DH5α, Bacillus subtilis and Corynebacterium glutamicum to improve the stability of indigo production. The fmo gene(1686bp) was amplified and subcloned into the vectors such as PHY300PLK, pEKEx2, pHCEIIB, pGEX4T-1 and PBSK. The recombinant plasmids produced indigo such as 182 mg/L (B. subtilis/pHY300PLK), 377mg/L (C. glutamicum/pEKEx2), 672.3 mg/L (E. coli DH5α/ pBSK SKII), 400.2 mg/L (E. coli DH5α/pHCE II B) and 638.5 mg/L (E. coli DH5α/pGEX4T-1), respectively.

키워드

저자정보

  • Gui Hwan HAN Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759.
  • Seong Hun IM Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759.
  • Jang Min PARK Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759.
  • Si Wouk KIM Dept. of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759.

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자료제공 : 네이버학술정보

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